Tretyakova I.N., Barsukova A.V.
 

V.N. Sukachev Institute of Forest SB RAS
660036 Krasnoyarsk, Russia, 50 Akademgorodok, 50; е-mail: culture@ksc.krasn.ru
 Volume XXVII, Number 1-2, 2010

Abstract

 As a result of experiments of hybridization of Pinus sibirica on clonally grafting plantation, cones of first generation with high seed productivity were obtained. Embrional suspensor masses, somatic embryo and plant regeneration from zygotic embryos of Siberian pine and Siberian larch in culture in vitro by selection of composition of culture medium have been obtained. Embrional masses of hybrid seeds from heterosis trees with annual cycle development of female cone have active growth. Genotypes of donor plants of Siberian larch and Siberian pine witch produced pure embryonal line, somatic embryo and plant have been determined.

Keywords: somatic embryo, embryonal mass, heterosis tree, Siberian larch, Siberian pine

 References 

Klimaszewska, K. Conifer somatic embryogenesis: I. Development / K. Klimaszewska, D.R. Cyr // Dendrobiology. – 2002. – V.48. – Р. 31-39.

Lelu, M.A. An improved method for somatic plantlet production in hybrid larch (Larix x leptoeuropaea): Part 2. Control for germination and plantlet development / M.A. Lelu, C. Bastien, K. Klimaszewska, P.J. Charest // Plant Cell Tiss. Org. Cult. – 1994. – V.36. - Р. 117-127.

Lelu-Walter, M-A. Clonal plant production from self- and cross-pollinated seed families of Pinus sylvestris (L.) through somatic embryogenesis / M-A. Lelu-Walter, M. Bernier-Cardou, K. Klimaszewska // Plant Cell Tiss Organ Cult. – 2008. – V.92. – Р. 31–45.

Murashige, T. A revised medium for rapid growth and bioassays with tobacco tissue cultures / T. Murashige, F. Skoog // Physiol. Plant. – 1962. – V.15. - № 4. – Р. 473-497.

Park, Y-S. Implementation in conifers somatic embryogenesis in clonal forestry: technical requirement and development considerations / Y-S. Park // Ann. For. Sci. – 2002. – V.59. – P. 651-656.

Plant cell, tissue and organ culture: fundamental methods / Eds. O.L. Gamborg, G.C.Phillips. Berlin: Springer-Velag, 1995, 358 p.

Белоруссова, А.С. Третьякова И.Н. Особенности формирования соматических зародышей у лиственницы сибирской: эмбриологические аспекты/ А.С. Белоруссова, И.Н. Третьякова // Онтогенез. - 2008. - Т. 39. - № 2 - С. 1-10.

Третьякова, И.Н. Перспективы применения методов биотехнологии для размножения генетически ценных форм лесных древесных видов / И.Н. Третьякова, А.С. Белоруссова, Н.Е. Носкова, С.С. Савельев, А.В. Лукина, А.В. Барсукова, М.В. Ижболдина, Ю.А. Череповский // Хвойные бореальной зоны. - 2007. - Т.24. - №2-3. - С. 309-318.