Abstract

 Induction of embryogenic callus and the getting somatic embryos of Siberian pine has been conducted with megagametophytes and immature zygotic embryos at different stages its development. Culturing was made on MS medium with hormones 2,4-D, 6-BA and ABA. Success of somatic embryogenesis of Siberian pine connected with tree genotypes and depends on the stage of explants development.

 

Key words: somatic embryo, embryogenic callus, megagametophyte

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