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Conifers of the boreal area

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Preservation of gene pool of Siberian conifer species by somatic embryogenesis in vitro – 
modern method of biotechnology
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 Tretyakova I.N., Barsukova A.V.
 

V.N. Sukachev Institute of Forest SB RAS
660036 Krasnoyarsk, Russia, 50 Akademgorodok, 50;
е-mail: culture@ksc.krasn.ru
 
Volume XXVII, Number 1-2, 2010

Abstract

 As a result of experiments of hybridization of Pinus sibirica on clonally grafting plantation, cones of first generation with high seed productivity were obtained. Embrional suspensor masses, somatic embryo and plant regeneration from zygotic embryos of Siberian pine and Siberian larch in culture in vitro by selection of composition of culture medium have been obtained. Embrional masses of hybrid seeds from heterosis trees with annual cycle development of female cone have active growth. Genotypes of donor plants of Siberian larch and Siberian pine witch produced pure embryonal line, somatic embryo and plant have been determined.

Keywords: somatic embryo, embryonal mass, heterosis tree, Siberian larch, Siberian pine

 References 

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