Abstract

 

Phytochemical investigation of extracts of fruiting bodies of the wood-rotting fungi honey mushroom Armillaria mellea (Vahl ex. Fr.) P. Kumm. led to the isolation and identification of several biologically active substantions, such as fatty acids, terpenoides, sterols, polyprenols, dolichols, pigments and other compounds. In China honey mushroom is eaten as food by people and used to treat of dizzness, headache, neurasthenia, insomnia, numbness in limbs, and convulsion. But information on the dominant components of extracts of Armillaria cepistipes Velen. is absent.

We obtainted MTBE-extract of fruiting bodies of Armillaria cepistipes and separated it towards neutral and acid constituents.

The neutral constituents were analyzed without the treatment (acetylation and silylation) using a Hewlett-Packard G 1800 A system with mass-selective detector HP 5971 and HP-5 MS column (30 m´ 0,25 mm ´ 0,25 μm). The investigation of the acid fractions was carried out after full methylation by diazomethane on the same conditions.

Our conditions of analysis permit to identify a row of substances such as aliphatic acids, hydrocarbons and alcohols, and to investigate some di- and triterpenic compounds. Erythritol was obtained selective. The most of these biologically active substances were found for the first time in this raw material. The obtained data were different from constituents of Armillaria mellea extracts.

 

Key words: chromatomass-spectrometry, lipophylic components, Armillaria, Honey Mushroom

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